Wee1 (D10D2) Rabbit mAb #13084
- WB
- IP
- IHC
- F
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #53692.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Wee1 is inactivated upon mitotic entry by phosphorylation at Ser53 and Ser123 by Plk1 and cdc2, followed by β-TrCP-mediated ubiquitination and degradation (1,9,10).
- Watanabe, N. et al. (1995) EMBO J 14, 1878-91.
- Hunter, T. (1995) Cell 80, 225-36.
- Galaktionov, K. et al. (1995) Genes Dev 9, 1046-58.
- McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
- Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6.
- Palmer, A. et al. (1998) EMBO J 17, 5037-47.
- Nakajima, H. et al. (2003) J Biol Chem 278, 25277-80.
- Parker, L.L. et al. (1995) Proc Natl Acad Sci U S A 92, 9638-42.
- Watanabe, N. et al. (2004) Proc Natl Acad Sci U S A 101, 4419-24.
Limited Uses
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