N-Cadherin (D4R1H) XP® Rabbit mAb #13116
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| IHC Leica Bond | 1:25 - 1:100 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Frozen) | 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #84117.
Protocol
Specificity / Sensitivity
N-Cadherin (D4R1H) XP® Rabbit mAb recognizes endogenous levels of total N-cadherin protein.
Some non-specific staining has been observed in mouse kidney tissue. Nonspecific labeling in fixed frozen mouse small intestine and lung has been observed by immunofluorescence.
Species Reactivity:
Source / Purification
Background
- Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
- Christofori, G. (2003) EMBO J 22, 2318-23.
- Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
- Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
- Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
- Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
- Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
- Sanders, D.S. et al. (2000) J Pathol 190, 526-30.
Limited Uses
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