Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb #19807
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Mk Z Dg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 92 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Z-Zebrafish
- Dg-Dog
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:500 - 1:2000 |
| Immunofluorescence (Frozen) | 1:1600 - 1:6400 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:3200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #24710.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb specifically recognizes β-catenin that is non-phosphorylated at Ser45. Non-phospho β-Catenin (Ser45) protein is useful as a readout of stabilized β-catenin protein, and therefore functionally active in cell-cell adhesion and mediating transcriptional activity via the canonical Wnt signaling pathway (9).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
- Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
- Amit, S. et al. (2002) Genes Dev 16, 1066-76.
- Liu, C. et al. (2002) Cell 108, 837-47.
- Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
- Yost, C. et al. (1996) Genes Dev 10, 1443-54.
- Morin, P.J. et al. (1997) Science 275, 1787-90.
- Sakanaka, C. (2002) J Biochem 132, 697-703.
Limited Uses
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