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Render Timestamp: 2024-11-27T11:14:38.763Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-08-01 15:31:06.636
Product last modified at: 2024-11-19T10:15:08.416Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. Phospho-S6 Ribosomal Protein (Ser235/236) Antibody

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211

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  • WB
  • IP
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY H M R Mk Sc
    SENSITIVITY Endogenous
    MW (kDa) 32
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Sc-S. cerevisiae 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:300 - 1:1200
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. This antibody does not detect ribosomal protein S6 phosphorylated at other sites.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, S. cerevisiae

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Chicken, Xenopus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).

    Database Links

    UniProt ID: P62753


    Entrez-Gene Id: 6194


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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