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  1. Products /
  2. Primary Antibodies /
  3. HER2/ErbB2 Antibody

HER2/ErbB2 Antibody #2242

Filter:
  • WB
Western Blotting Image 1: HER2/ErbB2 Antibody
Western blot analysis of extracts from MCF-7 and SKBR3 cells using HER2/ErbB2 Antibody.
1/1

To Purchase # 2242

Cat. # Size Qty. Price
2242S 100 µl
$350
2242L 300 µl
$736

Product Specifications

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 185
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HER2/ErbB2 Antibody detects endogenous levels of total ErbB2 protein. This antibody does not cross-react with related kinases.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1222 of human ErbB2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).

Alternate Names

c-erb B2/neu protein; CD340 antigen; erbB-2; HER2; herstatin; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P04626


Entrez-Gene Id: 2064


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