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Bax Antibody #2772

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  • IP
Western blot analysis of extracts from control HeLa cells (lane 1) or Bax knockout HeLa cells (lane 2) using Bax Antibody #2772 (upper), or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Bax-knockout HeLa cells confirms specificity of the antibody for Bax.

To Purchase # 2772

Cat. # Size Qty. Price
2772T 20 µl
$131
2772S 100 µl
$306

Product Specifications

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 20
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Bax Antibody detects endogenous levels of total Bax protein. This antibody does not cross-react with other Bcl-2 family members.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human Bax. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9, and initiation of the caspase activation pathway for apoptosis (3,4).

Alternate Names

Bax

Pathways

Explore pathways related to this product.


Product #2772
Citations: 926

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Showing 58 Images from 926 Citations
Showing 926 Citations
Journal:
J Cell Biol
Author:
Daniel Caballero, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Nature
Author:
Xufei Zhou, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Adv Sci (Weinh)
Author:
Zheng Zhong, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Nature
Author:
Jaya Gautam, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Genome Biol
Author:
Kangkang Niu, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
For Research Use Only. Not for Use in Diagnostic Procedures.
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