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Primary Antibodies /
Phospho-MAPKAPK-2 (Thr334) Antibody

Phospho-MAPKAPK-2 (Thr334) Antibody #3041

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Product Name

3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb

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Inquiry Info. # 3041

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Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	49
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-MAPKAPK-2 (Thr334) Antibody detects endogenous levels of MAPKAPK-2 protein phosphorylated at threonine 334. It may also cross-react with MAPKAPK-3 phosphorylated at threonine 313.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

Database Links

UniProt ID: P49137

Entrez-Gene Id: 9261

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For Research Use Only. Not For Use In Diagnostic Procedures.

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