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Render Timestamp: 2024-11-28T12:23:10.569Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-09-30 01:54:56.685
Product last modified at: 2024-11-21T12:45:29.042Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. NQO1 (A180) Mouse mAb

NQO1 (A180) Mouse mAb #3187

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 29
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:50
    Immunofluorescence (Immunocytochemistry) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NQO1 (A180) Mouse mAb detects endogenous levels of total NQO1 protein.

    Species Reactivity:

    Human

    Source / Purification

    NQO1 (A180) Mouse mAb is produced by immunizing mice with purified recombinant human NQO1.

    Background

    NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQO1 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow (2).

    Database Links

    UniProt ID: P15559


    Entrez-Gene Id: 1728


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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