Hoechst 33342 #4082
- IF
- F
Product Information
Product Usage Information
This product is soluble up to 50 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml.
Storage
Fluorescent Properties
- Free dye excitation maximum = 340 nm
- Free dye emission maximum = 510 nm
- DNA complex excitation maximum = 355 nm
- DNA complex emission maximum = 465 nm
Product Description
| Molecular Formula | C27H28N6O 3(HCl) 3(H2O) |
Background
Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. This stain can be used on both live and fixed cells (1).
Limited Uses
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