TRIM29/ATDC (E1L4E) Rabbit mAb #50292

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	68
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TRIM29/ATDC (E1L4E) Rabbit mAb recognizes endogenous levels of total TRIM29/ATDC protein. This antibody also cross-reacts with an unidentified protein of 49 kDa.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln499 of human TRIM29/ATDC protein.

Background

Tripartite motif-containing protein 29 (TRIM29, ATDC) was isolated as a candidate gene by its ability to complement the radiosensitivity defect of an ataxia-telangiectasia (AT) cell line (1). This putative transcription regulator belongs to the TRIM (tripartite motif) protein family that is characterized by highly conserved amino-terminal RING finger, B-box, and coiled-coil domains. The TRIM29 protein binds and sequesters cytosolic p53, repressing expression of p53 target genes including p21 and Noxa by preventing p53 from entering the nucleus. Expression of TRIM29 inhibits p53 function and results in increased cell proliferation. (2). TRIM29 enhances tumor growth and metastasis in vivo and high TRIM29 levels are seen in most invasive pancreatic cancers. The oncogenic effect of TRIM29 appears to require β-catenin as expression of both proteins is elevated in pancreatic cancer cell lines and tissues (3).

Database Links

UniProt ID: Q14134

Entrez-Gene Id: 23650

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