Semaphorin-4D/CD100 (E5C3B) XP® Rabbit mAb #53108
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| IHC Leica Bond | 1:200 |
| Immunohistochemistry (Paraffin) | 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #61515.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Sema4D has also been implicated in oncogenesis as research studies have demonstrated overexpression in multiple types of solid tumors (11,12). The role of Sema4D in oncogenesis, in part, has been linked to its ability to promote tumor angiogenesis (13), cell invasion (14), and immunosuppression through enhancement of myeloid derived suppressor cell function (15).
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- Elhabazi, A. et al. (1997) J Biol Chem 272, 23515-20.
- Kolodkin, A.L. et al. (1993) Cell 75, 1389-99.
- Bougeret, C. et al. (1992) J Immunol 148, 318-23.
- Hall, K.T. et al. (1996) Proc Natl Acad Sci U S A 93, 11780-5.
- Furuyama, T. et al. (1996) J Biol Chem 271, 33376-81.
- Kumanogoh, A. et al. (2000) Immunity 13, 621-31.
- Hérold, C. et al. (1995) Int Immunol 7, 1-8.
- Jiang, X. et al. (2017) Front Immunol 8, 765.
- Elhabazi, A. et al. (2001) J Immunol 166, 4341-7.
- Chen, Y. et al. (2012) Int J Mol Sci 13, 13264-74.
- Liu, H. et al. (2014) Microvasc Res 93, 1-8.
- Basile, J.R. et al. (2006) Proc Natl Acad Sci U S A 103, 9017-22.
- Kato, S. et al. (2011) Cancer Sci 102, 2029-37.
- Younis, R.H. et al. (2016) J Immunol 196, 1419-29.
Limited Uses
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