Caspase-3 Activity Assay Kit #5723
Product Information
Protocol
Product Description
Specificity / Sensitivity
Species Reactivity:
Background
Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (3-6). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates, such as PARP (3,5). During apoptosis, caspase-7 is activated by upstream caspases through proteolytic processsing at Asp23, Asp198, and Asp206, thereby producing the mature subunits (3,5). Similar to caspases-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (7).
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- Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
- Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
- Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11.
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