WT1 (D8I7F) XP® Rabbit mAb #83535
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 51-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
WT1 has a myriad of biological functions and a host of interacting partners and target genes (6). It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor (7). It exerts influence over the epigenetic landscape, and also has post translational influence of gene expression through RNA interactions (8). The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post translation modifications of the protein (9).
- Royer-Pokora, B. et al. (2004) Am J Med Genet A 127A, 249-57.
- Kohsaka, T. et al. (1999) Hum Mutat 14, 466-70.
- Little, M. et al. (2000) Hum Mutat 15, 389.
- Takata, A. et al. (2000) J Med Genet 37, 698-701.
- Suri, M. et al. (2007) Am J Med Genet A 143A, 2312-20.
- Toska, E. and Roberts, S.G. (2014) Biochem J 461, 15-32.
- Yang, L. et al. (2007) Leukemia 21, 868-76.
- Weiss, T.C. and Romaniuk, P.J. (2009) Biochemistry 48, 148-55.
- Haber, D.A. et al. (1991) Proc Natl Acad Sci U S A 88, 9618-22.
Limited Uses
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