PD-1 (Intracellular Domain) (D7D5W) XP® Rabbit mAb #84651
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40-75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #55789.
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
- Ishida, Y. et al. (1992) EMBO J 11, 3887-95.
- Shinohara, T. et al. (1994) Genomics 23, 704-6.
- Nishimura, H. et al. (1999) Immunity 11, 141-51.
- Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
- Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17.
- Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41.
- Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54.
- Thibult, M.L. et al. (2013) Int Immunol 25, 129-37.
- Dong, H. et al. (2002) Nat Med 8, 793-800.
- Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.
Limited Uses
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