Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb #8699
- WB
- IHC
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 24 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Phosphorylation at Thr35 by MST1/2 stabilizes MOB1, enhancing its binding and regulation of LATS1 (5). The resultant increase in LATS1 kinase activity promotes inhibitory phosphorylation of the transcriptional co-activators YAP and TAZ (11,12), leading to changes in the expression of genes involved in cell cycle progression (13).
- Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
- Edgar, B.A. (2006) Cell 124, 267-73.
- Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
- Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
- Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
- Praskova, M. et al. (2008) Curr Biol 18, 311-21.
- Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
- Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
- Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
- Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.
- Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
- Lei, Q.Y. et al. (2008) Mol Cell Biol 28, 2426-36.
- Hao, Y. et al. (2008) J Biol Chem 283, 5496-509.
Limited Uses
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