Render Target: STATIC
Render Timestamp: 2024-12-27T12:12:34.722Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:30:04.507
Product last modified at: 2024-12-17T22:15:15.485Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. SAPK/JNK Antibody

SAPK/JNK Antibody #9252

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Hm Mk Z B Sc
    SENSITIVITY Endogenous
    MW (kDa) 46, 54
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • Z-Zebrafish 
    • B-Bovine 
    • Sc-S. cerevisiae 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SAPK/JNK Antibody detects endogenous levels of total JNK1, JNK2 or JNK3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Zebrafish, Bovine, S. cerevisiae

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a recombinant human JNK2 fusion protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

    Database Links

    UniProt ID: P45983


    Entrez-Gene Id: 5599


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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