Render Target: STATIC
Render Timestamp: 2024-11-27T12:09:43.552Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-08-01 15:28:32.370
Product last modified at: 2024-11-20T18:00:09.292Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-GSK-3α/β (Ser21/9) Antibody #9331

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk Z
    SENSITIVITY Endogenous
    MW (kDa) 46 GSK-3beta. 51 GSK-3alpha.
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Z-Zebrafish 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-GSK-3alpha/beta (Ser21/9) Antibody detects endogenous levels of GSK-3 only when phosphorylated at serine 21 of GSK-3alpha or serine 9 of GSK-3beta.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Zebrafish

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to the sequences of human GSK-3 alpha and beta. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
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