Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) #9417

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Supporting Data

REACTIVITY	All
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:2000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:800

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) is prepared by biotinylation of the antibody via primary amines. Biotinylated antibody is useful for detection or purification of tyrosine-phosphorylated proteins using avidin-biotin binding. This antibody does not cross-react with proteins phosphorylated at serine or threonine.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-Tyr-containing peptides . Antibody is purified by protein A chromatography.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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