Name: Phospho-MSK1 (Thr581) Antibody
Price: 144.00 USD
Availability: InStock

Filter:

WB
IP

Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	90
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-MSK1 (Thr581) Antibody detects endogenous levels of MSK1 only when phosphorylated at Thr581. This antibody does not cross-react with MSK1 phosphorylated at other sites, nor does it detect phosphorylated RSK1, RSK2 or RSK3.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr581 of human MSK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.

Database Links

UniProt ID: O75582

Entrez-Gene Id: 9252

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Phospho-MSK1 (Thr581) Antibody #9595