Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634
- WB
- IP
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Peptide ELISA (DELFIA) | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine in a hydrophobic motif (3). For example, Akt, a kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site preceded by Phe at -4 and -1 and followed by Tyr at +1 (4). RSK2, p70 S6 kinase and certain PKC isoforms also contain a similar consensus phosphorylation motif. Phosphorylation of these motifs is required for binding to 3-phosphoinositide-dependent kinase 1 (PDK1) (5-7). Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Monoclonal Antibody is a powerful tool for the characterization of phosphorylated PDK1 docking motifs and the identification of new proteins with PDK1 docking motifs.
- Pinna, L.A. and Ruzzene, M. (1996) Biochim Biophys Acta 1314, 191-225.
- Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
- Vanhaesebroeck, B. and Alessi, D.R. (2000) Biochem J 346 Pt 3, 561-76.
- Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
- Frödin, M. et al. (2000) EMBO J 19, 2924-34.
- Balendran, A. et al. (1999) J Biol Chem 274, 37400-6.
- Balendran, A. et al. (2000) J Biol Chem 275, 20806-13.
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