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Nitro-Tyrosine Antibody

Nitro-Tyrosine Antibody #9691

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Supporting Data

REACTIVITY	All
SENSITIVITY	Endogenous
MW (kDa)
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

To obtain optimal results with this antibody please use PVDF instead of nitrocellulose membranes.

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Nitro-Tyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. The antibody does not cross-react with unmodified tyrosine or with phospho-tyrosine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Species Reactivity:

All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic nitro-tyrosine-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2), and neurodegeneration (3). High levels of both superoxide and NO in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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