Render Target: STATIC
Render Timestamp: 2024-11-15T09:44:04.740Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:19:42.150
Product last modified at: 2024-11-08T22:30:10.101Z
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PDP - Template Name: Miscellaneous
PDP - Template ID: *******097325a

Protein A Agarose Beads #9863

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  • IP

    Product Information

    Product Usage Information

    Vortex tube briefly to resuspend the beads. Add 20 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.

    Storage

    Supplied as a 50% slurry in 18.5% ethanol solution. This product is stable for 12 months when stored at 4°C.

    Protocol

    Product Description

    Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.

    Product Specifications:
    Bead Diameter: 45-165 micron per bead
    Binding Capacity: 20 +/- 2 mg human IgG/ml
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.