Branched Ubiquitin Antibody Sampler Kit #33959
Product Information
Kit Usage Information
Protocols
- 3936: Western Blotting, Immunohistochemistry (Paraffin)
- 5621: Western Blotting
- 7074: Western Blotting
- 7076: Western Blotting
- 8081: Western Blotting
Product Description
Specificity / Sensitivity
Source / Purification
Background
Substrate proteins are linked to ubiquitin using seven distinct ubiquitin lysine residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48 and Lys63). Formation of a polyubiquitin chain occurs when a lysine residue of ubiquitin is linked to the carboxy-terminal glycine of another ubiquitin. Proteins polyubiquinated at specific lysine residues display a tendency to be targeted for different processes; K48-linked polyubiquitin chains mainly target proteins for proteasomal degradation while K63-linked polyubiquitin regulates protein function, subcellular localization, or protein-protein interactions (8). K63-linked polyubiquitin chains exert nonproteolytic functions in vivo, such as protein trafficking, kinase/phosphatase activation, and DNA damage control, all of which might be important in regulation of cancer survival and development (9,10).
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- Hochstrasser, M. (2000) Science 289, 563-4.
- Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
- Aberle, H. et al. (1997) EMBO J 16, 3797-804.
- Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
- Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.
- Komander, D. (2009) Biochem Soc Trans 37, 937-53.
- Chen, Z.J. and Sun, L.J. (2009) Mol Cell 33, 275-86.
- Yang, W.L. et al. (2010) Oncogene 29, 4493-503.
Limited Uses
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