Render Target: STATIC
Render Timestamp: 2024-11-08T11:03:16.143Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:58:20.257
Product last modified at: 2024-10-21T11:15:27.072Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

mNeonGreen Tag (E8E3V) Rabbit mAb #55074

Filter:
  • WB

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    mNeonGreen Tag (E8E3V) Rabbit mAb detects recombinant proteins containing mNeonGreen protein. The antibody recognizes mNeonGreen protein fused to either the amino or carboxy terminus of targeted proteins in transfected cells. mNeonGreen Tag (E8E3V) Rabbit mAb detects exogenously expressed mNeonGreen protein in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody does not cross-react with green fluorescent protein (GFP).

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu18 of mNeonGreen protein.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    mNeonGreen is a 220 amino acid, bright green fluorescent protein derived from Branchiostoma lanceolatum. mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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