Product Information

Kit Usage Information

Protocols

7074: Western Blotting
8418: Western Blotting, Immunoprecipitation (Magnetic)
13008: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin)
13619: Western Blotting, Immunoprecipitation (Magnetic)
14074: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
53749: Western Blotting, Immunoprecipitation (Agarose)
59971: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin)
70148: Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic, Chromatin IP-seq

Product Description

The Phospho-YAP/TAZ Antibody Sampler Kit uses phospho-specific and control antibodies to provide an economical means of detecting the phosphorylation of YAP and TAZ proteins at critical residues that are reported to regulate YAP and TAZ protein stability. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Phospho-YAP/TAZ Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-YAP (Ser127) (D9W2I) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser127. Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser397. Phospho-YAP (Ser109) (E5I9G) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser109. Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb recognizes endogenous levels of TAZ protein only when phosphorylated at Ser89. Due to epitope sequence similarities, Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb may weakly detect YAP protein, but only when YAP is phosphorylated at Ser127.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with recombinant protein corresponding to the carboxy terminus of human YAP protein and synthetic peptides corresponding to Ala200 of mouse TAZ protein and Asp362 of human TAZ protein. Phosphorylation-specific antibodies are produced by immunizing rabbits with synthetic phospho-peptides corresponding to Ser109, Ser127, and Ser397 of human YAP protein, and Ser89 of human TAZ protein.

Background

YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. YAP and TAZ are structurally and functionally similar, but exhibit differential patterns of expression among cells and tissues that suggest partially non-redundant functions (1). YAP and TAZ are dynamically regulated in response to internal and external cellular signals. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with transcription factors (e.g., TEA domain proteins) that regulate the transcription of genes that control proliferation and cell survival (2). The subcellular localization of YAP and TAZ is dynamically regulated by a kinase cascade that regulates the phosphorylation status of key residues within YAP and TAZ. Phosphorylation of YAP and TAZ (e.g., Ser109, Ser127, Ser397 in YAP; Ser89 in TAZ) results in their cytoplasmic translocation, sequestration by 14-3-3 proteins, and recruitment of the β-TrCP (SCF) ubiquitin ligase complex (3,4). This complex ubiquitinates YAP and TAZ, triggering their proteolytic degradation in the proteasome, thereby altering the transcription of genes that control proliferation and cell survival (3-5).

Database Links

UniProt ID: Q9GZV5 , P46937

Entrez-Gene Id: 25937 , 10413

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Phospho-YAP/TAZ Antibody Sampler Kit #52420