Ribosomal Protein L7a (E109) Antibody #2415
- WB
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 32 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Ribosomal protein L7a Antibody detects endogenous levels of total ribosomal protein L7a. Immunofluorescent analysis using this antibody shows exclusive localization of ribosomal protein L7a to nucleoli during ribosomal biosynthesis.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu109 of human ribosomal protein L7a. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Ribosomal protein L7a is a highly conserved ribosome protein localized to 60S ribosomal subunit (1). The protein has distinct domains that target the newly synthesized polypeptide to nucleus and the nucleoli, the site of ribosome biosynthesis (2). Ribosomal protein L7a can also interact with RNA in vitro through two distinct RNA-binding domains in the protein (3). Taken together, nucleolar localization and the ability to bind RNA suggests that ribosomal protein L7a may act as an important component for ribosome biosynthesis and function.
Limited Uses
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