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PDP - Template Name: Detection System Kit (7071,7072)
PDP - Template ID: *******e168270
  1. Products /
  2. WB & IP Reagents /
  3. Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody

Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody #7071

Inquiry Info. # 7071

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    Product Information

    Product Usage Information

    There are six basic steps in the Western blotting procedures with the Phototope®-HRP Western Blot Detection System.

    Polyacrylamide Gel Electrophoresis of Proteins: Separate the protein samples and molecular weight standards by polyacrylamide gel electrophoresis. Transfer: Transfer the protein to membrane by standard electroblotting. Block Membrane: Block to saturate nonspecific binding sites on the membrane. 1° Antibody: Incubate the membrane with the primary antibody. 2° Antibody: Incubate the membrane with HRP-linked anti-rabbit IgG and HRP-linked anti-biotin antibodies. Chemiluminescent Detection: Add LumiGLO® reagent and capture the emitted light on X-ray film.

    Storage

    7074 Anti-rabbit IgG, HRP-linked Antibody: Store at -20°C.
    7727 Biotinylated Protein Ladder Detection Pack: Store at -20°C.
    7003 20X LumiGLO® Reagent and 20X Peroxide: Store at 4°C.

    Product Description

    The Phototope-HRP Western Blot Detection System is designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film.

    Background

    Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

      Limited Uses

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      Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

      For Research Use Only. Not For Use In Diagnostic Procedures.
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