Render Target: STATIC
Render Timestamp: 2024-11-22T11:05:30.935Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:17:14.872
Product last modified at: 2024-11-18T18:00:08.427Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127

Filter:
  • WB

    Supporting Data

    REACTIVITY Rab
    SENSITIVITY
    MW (kDa)
    Source/Isotype Mouse
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Rab-Rabbit 

    Product Information

    Product Description

    Affinity purified mouse anti-rabbit IgG (Conformation Specific) HRP Conjugate antibody.

    Product Usage Information

    Recommended Antibody Dilution:

    1:2000

    Storage

    Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

    Protocol

    Specificity / Sensitivity

    Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. This antibody may cross react with denatured IgG at high concentrations.

    Species Reactivity:

    Rabbit

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

    Background

    Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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