Revision 1

#58883

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
120

Source/Isotype:
Rabbit IgG

UniProt ID:
#P08235

Entrez-Gene Id:
4306

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Mineralocorticoid Receptor (E9W1M) Rabbit Monoclonal Antibody recognizes endogenous levels of total mineralocorticoid receptor protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human mineralocorticoid receptor protein.

Background

Mineralocorticoid receptor (MR) is a steroid hormone receptor with structural and functional similarities to glucocorticoid receptor (GR). MR binds with high affinity to aldosterone and other mineralocorticoids as well as glucocorticoids (1-3). Upon ligand binding, MR undergoes conformational changes and enters the nucleus to bind to target mineralocorticoid response elements (MREs) (3-5). MR is also able to heterodimerize with GR and bind to hormone response elements on DNA in cells that express both receptors (6-8). Unlike the anti-inflammatory activity of GR, aldosterone and MR can promote cardiovascular inflammation (9,10). Since MR can utilize both aldosterone and glucocorticoids as ligands, selectivity of ligands is facilitated by tissue-specific expression of 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD). This enzyme converts cortisol to cortisone, a derivative with very little affinity to MR, thus providing a means for tissue-specific MR regulation (11,12). Deficiency of 11 beta-OHSD has shown to cause an excess of MR signaling resulting in hypertension (12,13). Antimineralocorticoid spirolactones have been used as synthetic steroids to induce a transcriptionally inactivated conformational change in MR to treat hypertension and sodium retention (14,15).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using Mineralocorticoid Receptor (E9W1M) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, MSTO-211H is higher for mineralocorticoid receptor expression than in other cell lines.
Western Blotting Image 1: Mineralocorticoid Receptor (E9W1M) Rabbit Monoclonal Antibody
Immunoprecipitation of mineralocorticoid receptor protein from MSTO-211 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Mineralocorticoid Receptor (E9W1M) Rabbit mAb. Western blot analysis was performed using Mineralocorticoid Receptor (E9W1M) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: Mineralocorticoid Receptor (E9W1M) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.