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Render Timestamp: 2024-11-22T11:45:38.448Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:19:14.026
Product last modified at: 2024-11-15T13:00:14.524Z
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PDP - Template Name: Cellular Dyes
PDP - Template ID: *******0000253
  1. Products /
  2. Buffers & Dyes /
  3. MitoTracker® Red CMXRos

MitoTracker® Red CMXRos #9082

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    Product Information

    Product Usage Information

    Each vial of MitoTracker® Red CMXRos contains 50 μg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 94.1 μl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into growth media at a concentration between 50-200 nM and incubation between 15-45 min at 37ºC. After incubation, fix the cells in ice-cold methanol for 15 min at -20ºC then rinse 3 times with PBS for 5 min. Proceed with immunostaining.

    Storage

    Store lyophilized solid at -20°C dessicated and protected from light. In lyophilized form, this reagent is stable for 12 months. Once reconstituted in DMSO, the solution can be stored at -20°C, protected from light and must be used within 2 weeks. Avoid freeze-thaw cycles.

    Product Description

    MitoTracker® Red CMXRos is well retained after fixation allowing for further sample processing and immunostaining. Excitation: 579 nm, Emission: 599 nm, Molecular Weight: 531.52

    Background

    MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.

      Limited Uses

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      For Research Use Only. Not For Use In Diagnostic Procedures.
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