Revision 3

#94845

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IHC-P, IF-IC, FC-FP, FC-L

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
40-60

Source/Isotype:
Rabbit IgG

UniProt ID:
#P80370

Entrez-Gene Id:
8788

Product Usage Information

Application Dilution
Immunohistochemistry (Paraffin) 1:50 - 1:200
Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800
Flow Cytometry (Live) 1:1800 - 1:7200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #47633.

Specificity/Sensitivity

DLK1 (F7O3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total DLK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human DLK1 protein.

Background

Delta-like-1 homolog (DLK1), also known as fetal antigen 1 (FA1) and preadipocyte factor 1 (pref-1), is a member of the epidermal growth factor (EGF)-like family of proteins, containing six tandem EGF-like repeats (1,2). DLK1 is a paternally expressed, imprinted gene that plays an important role in normal development and homeostasis maintenance of adipose tissue mass (3). DLK1-deficient mice display growth retardation, obesity, skeletal malformation, and increased serum lipid metabolites (4). It has been reported that the DLK1 ectodomain is shredded from the cell surface and inhibits adipocyte differentiation (5-7).

Humans and rodents express multiple DLK1 isoforms, which are either membrane-bound or contain an ADAM17/TACE cleavage site for release of the soluble ectodomain (8). As high DLK1 expression is pro-oncogenic in some contexts, differential isoform expression may promote cancer cell survival, with both the ectodomain and intracellular domain having distinct functions (9). Under hypoxic conditions, HIF proteins induce ADAM17/TACE cleavage and internalization of the DLK1 intracellular domain, which localizes to the nucleus and alters Akt and p53 signaling cascades in glioma (10). Hypoxia increases DLK1 expression, and phosphorylation of the DLK1 C-terminus at Tyr339 and Ser355 increases neuronal tumor sphere growth (11). Nuclear DLK1 directly interacts with tumor suppressor NCoR1, correlating with poor prognosis in non-small cell lung cancer (NSCLC) (12). DLK1 has emerged as a target for novel antibody drug conjugates (ADCs) in neuroblastoma and adrenocortical carcinoma (13,14).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo

Immunohistochemical analysis of paraffin-embedded human adrenal neuroblastoma using DLK1 (F7O3Q) Rabbit mAb.
Immunohistochemistry Image 1: DLK1 (F7O3Q) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded normal human testis using DLK1 (F7O3Q) Rabbit mAb.

Immunohistochemistry Image 2: DLK1 (F7O3Q) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded normal human ovary using DLK1 (F7O3Q) Rabbit mAb.

Immunohistochemistry Image 3: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo

Immunohistochemical analysis of paraffin-embedded normal human placenta using DLK1 (F7O3Q) Rabbit mAb.

Immunohistochemistry Image 4: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human adrenocortical carcinoma using DLK1 (F7O3Q) Rabbit mAb (left) or a DLK1 Antibody (right). These two antibodies detect unique, non-overlapping epitopes on human DLK1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 5: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human adrenal gland using DLK1 (F7O3Q) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 6: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded SiMa cell pellet (left, positive) or Calu-1 cell pellet (right, negative) using DLK1 (F7O3Q) Rabbit mAb.
Immunohistochemistry Image 7: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of SiMa cells (left, positive) and Calu-1 cells (right, negative) using DLK1 (F7O3Q) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Flow cytometric analysis of fixed/permeabilized Calu-1 cells (blue, negative) and CMK cells (green, positive) using DLK1 (F7O3Q) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Flow cytometric analysis of live Calu-1 cells (blue, negative) and CMK cells (green, positive) using DLK1 (F7O3Q) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: DLK1 (F7O3Q) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.