Revision 1
#9964
Store at -20C
mTOR Pathway Antibody Sampler Kit
1 Kit
(6 x 20 microliters)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
---|---|---|---|---|
Phospho-mTOR (Ser2481) Antibody | 2974 | 20 µl | 289 kDa | Rabbit |
mTOR (7C10) Rabbit mAb | 2983 | 20 µl | 289 kDa | Rabbit IgG |
Raptor (24C12) Rabbit mAb | 2280 | 20 µl | 150 kDa | Rabbit |
Rictor (53A2) Rabbit mAb | 2114 | 20 µl | 200 kDa | Rabbit IgG |
GβL (86B8) Rabbit mAb | 3274 | 20 µl | 37 kDa | Rabbit IgG |
Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb | 5536 | 20 µl | 289 kDa | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
Storage
Background
The regulatory associated protein of mTOR (Raptor) interacts with mTOR to mediate mTOR signaling to downstream targets (10,11). Raptor binds to mTOR substrates, such as 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated substrate phosphorylation (12,13). Binding of the FKBP12-rapamycin complex to mTOR inhibits mTOR-raptor interaction, which suggests a mechanism for the inhibition of mTOR signaling by rapamycin (14). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (15). GβL is part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR) and may mediate rictor-mTOR signaling to PKCα and other downstream targets (16). The rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB at Ser473, which is required for PDK1 phosphorylation of Akt/PKB at Thr308 and full activation of Akt/PKB (17).
Background References
- Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
- Brown, E.J. et al. (1994) Nature 369, 756-8.
- Sabatini, D.M. et al. (1994) Cell 78, 35-43.
- Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
- Dennis, P.B. et al. (2001) Science 294, 1102-5.
- Fang, Y. et al. (2001) Science 294, 1942-5.
- Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
- Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
- Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.
- Hara, K. et al. (2002) Cell 110, 177-189.
- Kim, D.H. et al. (2002) Cell 110, 163-175.
- Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-40722.
- Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-15464.
- Oshiro, N. et al. (2004) Genes Cells 9, 359-366.
- Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
- Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
- Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
Trademarks and Patents
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Limited Uses
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