Revision 1
#31953
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
FC-L
Reactivity:
H M
Sensitivity:
Endogenous
Source/Isotype:
Rat IgG2a kappa
UniProt ID:
#P06800
Entrez-Gene Id:
19264
Product Usage Information
For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.
Application | Dilution |
---|---|
Flow Cytometry (Live) | 1:80 |
Storage
Specificity/Sensitivity
CD45R/B220 (RA3-6B2) Rat mAb (APC-Cy7® Conjugate) recognizes endogenous levels of total CD45R/B220protein. This antibody detects an epitope within the extracellular domain.
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Description
This Cell Signaling Technology antibody is conjugated to APC-Cy7® and tested in-house for direct flow cytometric analysis in human cells.
Background
The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).
The RA3-6B2 clone is raised against an isoform of CD45 known as CD45R/B220. CD45R/B220 is widely used as a marker for B cells, T cell subsets, and NK cell subsets in both human and mouse (5).
Background References
- Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74.
- Felberg, J. and Johnson, P. (2000) Biochem Biophys Res Commun 271, 292-8.
- Kashio, N. et al. (1998) J Biol Chem 273, 33856-63.
- Wang, Y. and Johnson, P. (2005) J Biol Chem 280, 14318-24.
- Hermiston, M.L. et al. (2003) Annu Rev Immunol 21, 107-37.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cy and CyDye are registered trademarks of GE Healthcare.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
Flow cytometric analysis of live mouse splenocytes using CD45R/B220 (RA3-6B2) Rat mAb (APC-Cy7® Conjugate) (solid line) compared to concentration-matched Rat Isotype Control (APC-Cy7® Conjugate) (dashed line).