Revision 1
#48505
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
IF-IC, FC-FP
Reactivity:
H M
Sensitivity:
Endogenous
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q02548
Entrez-Gene Id:
5079
Product Usage Information
Application | Dilution |
---|---|
Immunofluorescence (Immunocytochemistry) | 1:50 |
Flow Cytometry (Fixed/Permeabilized) | 1:800 |
Storage
Specificity/Sensitivity
PAX5 (D7H5X) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PAX5 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PAX5 protein.
Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated PAX5 (D7H5X) XP® Rabbit mAb #12709.
Background
Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).
PAX5, also known as B cell-specific activator protein (BSAP), was originally identified as a DNA-binding protein with affinity for both immunoglobulin heavy-chain and kappa light-chain loci (5). PAX5 is unique within the PAX family in being the only member with reported expression in the hematopoietic system. PAX5 is required to promote differentiation of common lymphoid progenitors (CLPs) into B cells (5,6); it is also required for the continued maintenance of B cell identity following differentiation (7). Disruptions to the expression of PAX5 have consequently been linked with lymphoid cancer development (8).
Background References
- Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
- Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
- Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
- Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
- Cobaleda, C. et al. (2007) Nat Immunol 8, 463-70.
- Busslinger, M. (2004) Annu Rev Immunol 22, 55-79.
- Carotta, S. and Nutt, S.L. (2008) Bioessays 30, 203-7.
- Heltemes-Harris, L.M. et al. (2011) J Exp Med 208, 1135-49.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
XP is a registered trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
Confocal immunofluorescent analysis of Ramos cells (left, positive) and Jurkat cells (right, negative) using PAX5 (D7H5X) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (red), COX IV (4D11-B3-E8) Mouse mAb #11967 (green), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Flow cytometric analysis of fixed/permeabilized human peripheral blood mononuclear cells using PAX5 (D7H5X) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (left), co-stained with CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb (PE Conjugate) #58960.