Revision 1
#80435
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For Research Use Only. Not for Use in Diagnostic Procedures.
MW (kDa):
17-20
UniProt ID:
#P01562
Entrez-Gene Id:
3439
Background
Interferon-α1 is a member of the type I interferon (IFN) (1) family best known for their antiviral activity. Most nucleated cells produce one or more type I IFNs in response to viral infection (2). Secreted type I IFN then induces viral protective responses in neighboring non-infected cells. Type I IFNs also enhance virus-induced apoptosis (3). Other IFN-α1 activities include enhancement of dendritic cell maturation and cytotoxic T cell activity (4). IFN-α1 binds to the IFN-αR1 and IFN-αR2 heterodimer (1). Intracellular signaling through the Jak/Stat pathway is best characterized (3). However, the PI3K, ERK, and p38 kinase pathways are also involved (5). The antiviral activities of the IFNs have led to their use in treating viral infections (4). Type I IFNs also appear to have an integral role in several autoimmune diseases (6).
Endotoxin
Endotoxin levels are less than or equal to 1 EU/ 1 μg hIFN-α1.
Purity
A greater than or equal to 95% purity was determined by SDS-PAGE.
Source / Purification
Recombinant human IFN-α1 was expressed in 293 cells with a C-terminal His-tag and is supplied in a lyophilized form.
Bioactivity
The bioactivity of recombinant IFN-α1 was determined in a TF-1 human erythroleukemic cell proliferation assay. The ED50 for this effect is 0.327 ng/mL.
Background References
- Nagai, T. et al. (2003) J Immunol 171, 5233-43.
- Stetson, D.B. and Medzhitov, R. (2006) Immunity 25, 373-81.
- Vilcek, J. (2006) Immunity 25, 343-8.
- Luft, T. et al. (2002) Int Immunol 14, 367-80.
- van Boxel-Dezaire, A.H. et al. (2006) Immunity 25, 361-72.
- Banchereau, J. and Pascual, V. (2006) Immunity 25, 383-92.
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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