Revision 4
#7204
Store at +4C
PathScan® Acetyl-α-Tubulin Sandwich ELISA Kit
1 Kit
(96 assays)
Species Cross Reactivity:
H M Mk
UniProt ID:
#Q71U36
Entrez-Gene Id:
#7846
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes | Product # | Quantity | Color | Storage Temp |
---|---|---|---|---|
Tubulin Mouse mAb Microwells | 77463 | 96 tests | +4C | |
Acetylated-Lysine Rabbit Detection mAb | 13968 | 1 ea | Green (Lyophilized) | +4C |
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) | 13272 | 1 ea | Red (Lyophilized) | +4C |
Detection Antibody Diluent | 13339 | 11 ml | Green | +4C |
HRP Diluent | 13515 | 11 ml | Red | +4C |
TMB Substrate | 7004 | 11 ml | +4C | |
STOP Solution | 7002 | 11 ml | +4C | |
Sealing Tape | 54503 | 2 ea | +4C | |
ELISA Wash Buffer (20X) | 9801 | 25 ml | +4C | |
ELISA Sample Diluent | 11083 | 25 ml | Blue | +4C |
Cell Lysis Buffer (10X) | 9803 | 15 ml | -20C |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
CST's PathScan® Acetyl-α-Tubulin Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetylated α-tubulin protein. An α-tubulin mouse mAb has been coated onto the microwells. After incubation with cell lysates, α-tubulin protein (acetylated and non-acetylated) is captured by the coated antibody. Following extensive washing, an acetyl-lysine rabbit Ab is added to detect the acetylated α-tubulin protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of acetylated α-tubulin protein.
*Antibodies in this kit are custom formulations specific to kit.
Specificity/Sensitivity
CST's PathScan® Acetyl-α-Tubulin Sandwich ELISA Kit #7204 detects endogenous levels of acetylated α-tubulin. As shown in Figure 1 using the Acetyl-α-Tubulin Sandwich ELISA Kit #7204, a high level of acetylated α-tubulin is detected in COS cells when treated with TSA. The level of total α-tubulin (acetylated and non-acetylated) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when NIH/3T3 and Jurkat cells are treated with TSA (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Background References
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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