The PathScan^® Tau (GT-38) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of a conformational selective form of tau protein related to Alzheimer's disease. A Tau (GT-38) Mouse mAb has been coated onto the microwells. After incubation with cell lysate, the conformational selective form of tau protein is captured by the coated antibody. Following extensive washing, a biotinylated tau rabbit detection antibody is added to detect the captured conformational selective form of tau protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of a conformational selective form of tau protein.

*Antibodies in this kit are custom formulations specific to kit.

The PathScan^® Tau (GT-38) Sandwich ELISA Kit utilizes a Tau (GT-38) Mouse mAb that recognizes paired helical filament conformational tau protein. This antibody preferentially recognizes tau conformations related to Alzheimer's disease compared to other tauopathies. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Alternative splicing of exon 10 results in the expression of two groups of tau: three-repeat and four-repeat tau. Isoforms 2, 4, and 5 express three microtubule binding repeat domains (Tau 3R), while isoforms 6, 7, and 8 express four microtubule binding repeat domains (Tau 4R) (4). Expression of Tau 3R and Tau 4R in cells can be different in mild or pathological conditions. For example, Tau 3R is preferentially expressed in Pick's disease (PiD) and corticobasal degeneration (CBD), while Tau 3R and Tau 4R are equally expressed in AD (5,6). The repeat-dependent tau has a different pattern of phosphorylation in different diseases, and also has the ability and patterns of aggregation (7-9). These varying patterns of aggregation result in disease-specific conformational structures, including hyperphosphorylated straight filaments (SFs) and PHFs in AD compared to SFs and twisted filaments in both PiD and CBD (10,11).

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