Revision 1
#54036
Store at -20C
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H Mk
Sensitivity:
Endogenous
MW (kDa):
44
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q13686
Entrez-Gene Id:
8846
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
ALKBH1 (E6Q8I) Rabbit mAb recognizes endogenous levels of total ALKBH1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ALKBH1 protein.
Background
AlkB is an oxidative dealkylating DNA repair enzyme first characterized in E.coli (1-5). Nine AlkB homologs exist in mammals, with the first eight designated as ALKBH1-ALKBH8, and the ninth as FTO (fat mass and obesity-associated protein) (6). ALKBH1, which features the highest sequence identity to E.coli AlkB, is an Fe(II) and 2-oxoglutarate-dependent dioxygenase that acts upon nucleic acids such as DNA and tRNA and carries out a wide range of enzymatic functions (6,7). Similar to other AlkB proteins, ALKBH1 is able to repair alkylated single-stranded DNA and RNA containing 3-methylcytosine (m3C), albeit with weak activity (8). Perhaps more importantly, it has also been shown to catalyze the demethylation of N1-methyladenosine on tRNAs to regulate translation (9). ALKBH1 functions in the mitochondria as well, recognizing and oxidizing 5-methylcytosine (m5C) on mitochondrial tRNAMet to generate 5-formylcytosine, consequently enhancing mitochondrial translation (10). Interestingly, ALKBH1 has also been shown to possess apurinic/apyrimidinic (AP) lyase activity, cleaving both single-stranded and double-stranded DNA at abasic sites, with greatest affinity toward double-stranded DNA with two abasic sites (11). Lastly, ALKBH1 has been reported to possess N(6)-methyladenine (6mA) demethylase activity, suggesting a role in epigenetic regulation (12,13). However, an additional study was unable to show definitive ALKBH1 6mA demethylase activity using both biochemistry assays and knockout mice, so this enzymatic function remains controversial (9).
Background References
- Samson, L. and Cairns, J. (1977) Nature 267, 281-3.
- Chen, B.J. et al. (1994) J Bacteriol 176, 6255-61.
- Aravind, L. and Koonin, E.V. (2001) Genome Biol 2, RESEARCH0007.
- Trewick, S.C. et al. (2002) Nature 419, 174-8.
- Falnes, P.Ø. et al. (2002) Nature 419, 178-82.
- Fedeles, B.I. et al. (2015) J Biol Chem 290, 20734-42.
- Müller, T.A. et al. (2018) Biochem Biophys Res Commun 495, 98-103.
- Westbye, M.P. et al. (2008) J Biol Chem 283, 25046-56.
- Liu, F. et al. (2016) Cell 167, 816-828.e16.
- Haag, S. et al. (2016) EMBO J 35, 2104-19.
- Müller, T.A. et al. (2010) DNA Repair (Amst) 9, 58-65.
- Xiao, C.L. et al. (2018) Mol Cell 71, 306-318.e7.
- Wu, T.P. et al. (2016) Nature 532, 329-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#54036
ALKBH1 (E6Q8I) Rabbit mAb
Western blot analysis of extracts from various cell lines using ALKBH1 (E6Q8I) Rabbit mAb.