Render Target: STATIC
Render Timestamp: 2025-01-22T13:57:48.305Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-01 15:28:27.544
Product last modified at: 2025-01-01T09:07:50.382Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ANTXR1/TEM8 Antibody #23494

Filter:
  • WB
  • IP
Western Blotting Image 1: ANTXR1/TEM8 Antibody
Western blot analysis of extracts from various cell lines using ANTXR1/TEM8 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of ANTXR1/TEM8 expression in HT-29 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.

To Purchase # 23494

Cat. #

Size

23494S
100 µl

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 80
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ANTXR1/TEM8 Antibody recognizes endogenous levels of total ANTXR1/TEM8 protein.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg557 of human ANTXR1/TEM8 protein. Antibodies are purified by peptide affinity chromatography.

Background

ANTXR1 (Anthrax toxin receptor 1, TEM8, tumor endothelial marker 8) is a protein biomarker induced in tumor-associated endothelia and acts as a cell surface receptor for the anthrax toxin and the oncolytic Seneca Valley Virus (1-3). The protein is a type I transmembrane glycoprotein that is expressed on the epithelial cell surface of skin, lung, and intestine tissues as a docking site for toxin/virus binding and endocytosis, and in tumor endothelial cells for tumor-associated vascular development (4). ANTXR1 interacts with type I and type VI collagens in the extracellular matrix, and with actin cytoskeleton in the cytoplasm to promote tumor-associated endothelial cell adhesion, migration, and spreading during angiogenesis (4-6). Aberrant high expression of ANTXR1 has been found in various cancer types including metastatic breast cancer cells, pancreatic cancer stem cells, etc. (7-9); there the protein promotes tumor proliferation, growth, and invasion. Because of its specific high expression in tumors and to their surrounding vasculature, ANTXR1 has been proposed as a target for CAR T cell therapy for cancer treatment (10,11).
For Research Use Only. Not For Use In Diagnostic Procedures.
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