Revision 1

#53049

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-Bond, IHC-P

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
30

Source/Isotype:
Rabbit IgG

UniProt ID:
#P50553

Entrez-Gene Id:
429

Product Usage Information

This product is the carrier free version of product #10585. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #10585

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity/Sensitivity

ASCL1 (E5S4Q) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total ASCL1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro83 of human ASCL1 protein.

Background

Achaete-scute homolog 1 (ASCL1), also known as MASH-1, is a basic helix-loop-helix (BHLH) transcription factor which plays an essential role in the differentiation of neuroendocrine cells and neural tissues (1-3). ASCL1 directly binds the E-box motif (5'-CANNTG-3') on promoters, with dimerization with other BHLH proteins required for efficient DNA binding (4). Acting as a pioneer transcription factor, ASCL1 also accesses closed chromatin, allowing other factors to promote transcription of neuronal genes and activate neural pathways (5). Research studies have shown that knockdown of the ASCL1 gene leads to inhibition of growth and induction of apoptosis in SCLC cells in vitro (6). Additionally, ASCL1 is overexpressed in both classic SCLC as well as NSCLC with neuroendocrine features, suggesting its role in the pathogenesis of those malignancies (7). ASCL1 plays a crucial role in promoting SCLC carcinogenesis by upregulating the expression of DLL3, a nonfunctioning Notch ligand, leading to inhibition of the Notch signaling pathway (8).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IHC-Bond: IHC Leica Bond

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Western blot analysis of extracts from various cell lines using ASCL1 (E5S4Q) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of ASCL1 protein in U-2 OS and SK-MEL-28 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.

Western Blotting Image 1: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx.   Data were generated using the standard formulation of this product.

Immunohistochemistry Image 1: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 1: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 2: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the gallbladder using ASCL1 (E5S4Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 3: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human metastatic neuroendocrine carcinoma of the lung (two regions of interest) using ASCL1 (E5S4Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 4: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using ASCL1 (E5S4Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).  Data were generated using the standard formulation of this product.

Immunohistochemistry Image 5: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded NCI-H146 cell pellet (left, positive) or SK-MEL-28 cell pellet (right, negative) using ASCL1 (E5S4Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 6: ASCL1 (E5S4Q) XP<sup>®</sup> Rabbit mAb (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.