WB, IP, FC-L
H
Endogenous
240, 120
Rabbit IgG
#Q14242
6404
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Flow Cytometry (Live) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly281 of human CD162/PSGL-1 protein.
Background
P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is a disulfide-linked homodimer expressed on the cell surface of lymphoid and myeloid cells as a type I transmembrane sialoglycoprotein (1,2). The mature protein backbone of the extracellular domain of PSGL-1 is extensively modified with N-linked glycans, O-linked glycans, sulfated tyrosine residues, and sialyl-Lewis X epitopes (3). Collectively, these modifications modulate high affinity interactions between PSGL-1 and multiple selectins, which facilitates immune cell trafficking to primary and secondary lymphoid organs (4-8).
- Laszik, Z. et al. (1996) Blood 88, 3010-21.
- Snapp, K.R. et al. (1998) Blood 91, 154-64.
- Norgard, K.E. et al. (1993) J Biol Chem 268, 12764-74.
- Vachino, G. et al. (1995) J Biol Chem 270, 21966-74.
- Wilkins, P.P. et al. (1995) J Biol Chem 270, 22677-80.
- Borges, E. et al. (1997) J Biol Chem 272, 28786-92.
- Martinez, M. et al. (2005) J Biol Chem 280, 5378-90.
- Carlow, D.A. et al. (2009) Immunol Rev 230, 75-96.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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