Revision 4
#94135
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
IHC-Bond, IHC-P
Reactivity:
H
Sensitivity:
Endogenous
Source/Isotype:
Mouse IgG2b
UniProt ID:
#P09564
Entrez-Gene Id:
924
Product Usage Information
Application | Dilution |
---|---|
IHC Leica Bond | 1:100 - 1:400 |
Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Specificity/Sensitivity
CD7 (LP15) Mouse mAb recognizes endogenous levels of CD7 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain of human CD7 protein.
Background
CD7 is a type-I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is one of the earliest surface markers to be expressed on the surface of developing T cells and its expression is maintained throughout maturation of multiple T cell subsets and NK cells (1-3). Engagement of CD7 through binding its ligand, SECTM1, has been shown to promote tyrosine phosphorylation of its cytoplasmic domain, recruitment of PI3K, and delivery of costimulatory signals for T cell activation (4-6). While CD7 is expressed on normal T cells, it is also highly expressed in a variety of T cell malignancies, which has poised it as a potential target of immunotherapy (7-9).
Background References
- Haynes, B.F. et al. (1988) J Exp Med 168, 1061-80.
- Lobach, D.F. et al. (1985) J Immunol 135, 1752-9.
- Rabinowich, H. et al. (1994) J Immunol 152, 517-26.
- Ward, S.G. et al. (1995) Eur J Immunol 25, 502-7.
- Chan, A.S. et al. (1997) J Immunol 159, 934-42.
- Lyman, S.D. et al. (2000) J Biol Chem 275, 3431-7.
- Cooper, M.L. et al. (2018) Leukemia 32, 1970-1983. doi: 10.1038/s41375-018-0065-5.
- Png, Y.T. et al. (2017) Blood Adv 1, 2348-2360.
- Gomes-Silva, D. et al. (2017) Blood 130, 285-296.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-Bond: IHC Leica Bond
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
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