Revision 4
#19589
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IHC-Bond, IHC-P
Reactivity:
M
Sensitivity:
Endogenous
MW (kDa):
60-85
Source/Isotype:
Rabbit IgG
UniProt ID:
#P42082
Entrez-Gene Id:
12524
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
IHC Leica Bond | 1:200 - 1:800 |
Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #20018.
Specificity/Sensitivity
CD86 (E5W6H) Rabbit mAb recognizes endogenous levels of total CD86 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with mouse CD86 recombinant protein.
Background
CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
Background References
- Freeman, G.J. et al. (1989) J Immunol 143, 2714-22.
- Azuma, M. et al. (1993) Nature 366, 76-9.
- Adams, A.B. et al. (2016) J Immunol 197, 2045-50.
- Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5.
- Gimmi, C.D. et al. (1991) Proc Natl Acad Sci U S A 88, 6575-9.
- Harding, F.A. et al. (1992) Nature 356, 607-9.
- Collins, A.V. et al. (2002) Immunity 17, 201-10.
- Linsley, P.S. et al. (1991) J Exp Med 174, 561-9.
- Krummel, M.F. and Allison, J.P. (1995) J Exp Med 182, 459-65.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IHC-Bond: IHC Leica Bond
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 4
#19589
CD86 (E5W6H) Rabbit mAb
Western blot analysis of extracts from J774A.1 cells, Neuro-2a cells, and mouse spleen tissue using CD86 (E5W6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded mouse liver using CD86 (E5W6H) Rabbit mAb performed on the Leica® BOND™ Rx.
Immunohistochemical analysis of paraffin-embedded mouse lung using CD86 (E5W6H) Rabbit mAb performed on the Leica® BOND™ Rx.
Revision 4
#19589
CD86 (E5W6H) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using CD86 (E5W6H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded GL-261 syngeneic tumor using CD86 (E5W6H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded J774A.1 cell pellet (left, positive) or BA/F3 cell pellet (right, negative) using CD86 (E5W6H) Rabbit mAb.
Revision 4
#19589
CD86 (E5W6H) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD86 (E5W6H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).