Revision 1
#48501
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
23
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q16667
Entrez-Gene Id:
1033
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity/Sensitivity
CDKN3 (E7O5K) Rabbit mAb recognizes endogenous levels of total CDKN3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human CDKN3 protein.
Background
CDKN3 (Cyclin-Dependent Kinase Inhibitor 3)/KAP (Kinase-Associated Phosphatase) is a dual specificity phosphatase that regulates cell cycle progression through dephosphorylation and inhibition of cyclin-dependent kinases (1). CDKN3 promotes cancer cell proliferation and invasion (2-4), as well as resistance to cisplatin (5,6). Overexpression of CDKN3 is associated with tumor progression and poor prognosis in several human cancers (5-9).
Background References
- Hannon, G.J. et al. (1994) Proc Natl Acad Sci U S A 91, 1731-5.
- Deng, M. et al. (2016) Mol Med Rep 14, 1523-30.
- Li, Y. et al. (2017) Oncol Res 25, 721-731.
- Yu, H. et al. (2020) Oncol Lett 19, 542-548.
- Wang, J. et al. (2019) Cancer Manag Res 11, 3253-3264.
- Li, W.H. et al. (2020) Eur Rev Med Pharmacol Sci 24, 3614-3623.
- Dai, W. et al. (2020) J Int Med Res 48, 300060520936879.
- Fan, C. et al. (2015) Br J Cancer 113, 1735-43.
- Barrón, E.V. et al. (2015) PLoS One 10, e0137397.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#48501
CDKN3 (E7O5K) Rabbit mAb
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or CDKN3 siRNA (+), using CDKN3 (E7O5K) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from various cell lines using CDKN3 (E7O5K) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of CDKN3 protein from U-2 OS cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CDKN3 (E7O5K) Rabbit mAb. Western blot analysis was performed using CDKN3 (E7O5K) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.