Revision 1
#36276
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H M
Sensitivity:
Endogenous
MW (kDa):
333
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q99715
Entrez-Gene Id:
1303
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Collagen type XII α1 chain (COL12A1), the largest member of the fibril-associated collagens with interrupted triple helix (FACIT) collagen family, contains fibronectin type III repeats, von Willebrand factor A domains, and two triple-helical domains (4). There are two major isoforms, a long form of 3063 amino acids and a short form of 1899 amino acids. COL12A1 binds to type I collagen fibrils and contributes to tissue biomechanics in the cornea, skeletal muscle, and tendon tissues (reviewed in 5). Mutations in the COL12A1 gene are correlated with myopathapies (6,7). COL12A1 expression is upregulated in several types of cancer; this elevation is thought to enhance tumor migration, invasiveness, and metastasis and, in some cases, correlates with poor prognosis (8-13).
Background References
- Barkan, D. et al. (2010) Eur J Cancer 46, 1181-8.
- Hynes, R.O. (2009) Science 326, 1216-9.
- Ricard-Blum, S. (2011) Cold Spring Harb Perspect Biol 3, a004978.
- Gerecke, D.R. et al. (1997) Genomics 41, 236-42.
- Chiquet, M. et al. (2014) Int J Biochem Cell Biol 53, 51-4.
- Hicks, D. et al. (2014) Hum Mol Genet 23, 2353-63.
- Zou, Y. et al. (2014) Hum Mol Genet 23, 2339-52.
- Yan, Y. et al. (2023) Endocr Relat Cancer 30, e230012. doi: 10.1530/ERC-23-0012.
- Papanicolaou, M. et al. (2022) Nat Commun 13, 4587.
- Jiang, X. et al. (2019) Mol Med Rep 20, 3103-3112.
- Wu, Y. and Xu, Y. (2020) Cancer Med 9, 4743-4755.
- Duan, S. et al. (2018) Mol Med Rep 18, 3727-3736.
- Karagiannis, G.S. et al. (2012) Oncotarget 3, 267-85.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse
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