WB, IP
H M R Mk
Endogenous
47
Rabbit
#P56545
1488
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog, Guinea Pig
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CtBP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CtBP2 (carboxy-terminal binding protein-2) and its homolog CtBP1 are transcriptional co-repressors originally identified as proteins that bind the carboxy-terminus of the human adenovirus E1A protein (1-3). CtBP proteins are thought to play important roles in regulating various developmental pathways because deletion of CtBP2 leads to embryonic lethality at E10.5 and is correlated with axial patterning defects (4). CtBP proteins regulate various oncogenic signaling pathways as promoters of epithelial-mesenchymal transition, apoptosis antagonists, and tumor suppressor genes repressors (1,5). The CtBP protein transcription co-repression activity results from interactions with numerous transcription factors and chromatin modulators, including the polycomb group proteins (1,6,7). Depending on the context, CtBP proteins interact with a short amino acid sequence motif (PXDLS) to mediate repression of target genes through both histone deacetylase-dependent and independent mechanisms (6,8,9). CtBP proteins display a high sequence homology to the bacterial D-isomer-specific 2-hydroxyacid dehydrogenase enzymes. Research studies indicate that nuclear NADH levels regulate CtBP transcription repression activities, as NADH binding is required for CtBP2 homodimerization and transcription co-repressor activity (6,9-11).
- Chinnadurai, G. (2009) Cancer Res 69, 731-4.
- Boyd, J.M. et al. (1993) EMBO J 12, 469-78.
- Katsanis, N. and Fisher, E.M. (1998) Genomics 47, 294-9.
- Hildebrand, J.D. and Soriano, P. (2002) Mol Cell Biol 22, 5296-307.
- Battaglia, S. et al. (2010) Int J Cancer 126, 2511-9.
- Chinnadurai, G. (2002) Mol Cell 9, 213-24.
- Sewalt, R.G. et al. (1999) Mol Cell Biol 19, 777-87.
- Molloy, D.P. et al. (1998) J Biol Chem 273, 20867-76.
- Schaeper, U. et al. (1995) Proc Natl Acad Sci U S A 92, 10467-71.
- Kumar, V. et al. (2002) Mol Cell 10, 857-69.
- Thio, S.S. et al. (2004) Nucleic Acids Res 32, 1836-47.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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