Revision 1
#29113
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877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IHC-P, IF-IC, FC-FP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
55
Source/Isotype:
Rabbit IgG
UniProt ID:
#P20248
Entrez-Gene Id:
890
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Supplied in 1X PBS, BSA and Azide Free.
For standard formulation of this product see product #67955
Storage
Specificity/Sensitivity
Cyclin A2 (E6D1J) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total cyclin A2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human cyclin A2 protein. The epitope corresponds to a region surrounding Glu121 of human cyclin A2.
Background
While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of overexpressed cyclin A to accelerate the G1 to S transition, causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for the completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
Western blot analysis of extracts from HCT 116 cells, transfected with control siRNA (-) or cyclin A2 siRNA (+), using Cyclin A2 (E6D1J) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western blot analysis of extracts from various human cell lines, untreated (-) or treated with Aphidicolin #32774 (10 μg/mL, 24 hr; +) or Doxorubicin #5927 (0.5 μM, 24 hr; +), using Cyclin A2 (E6D1J) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Cyclin A2 protein is induced with aphidicolin and reduced with doxorubicin as expected. Low expression of cyclin A2 protein in Caki-1 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Cyclin A2 (E6D1J) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded normal human thymus using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, untreated (left) or treated with Aphidicolin #32774 (10 µg/ml, 24 hr; right), using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human large cell neuroendocrine carcinoma using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Revision 1
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human small cell carcinoma of the salivary gland using Cyclin A2 (E6D1J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of HCT 116 cells, either mock transfected (left, moderate-expressing), transfected with siRNA directed against human cyclin A2 (center, low-expressing), or treated with Aphidicolin #32774 (10 µg/mL, 24 hr; right, high-expressing), using Cyclin A2 (E6D1J) XP® Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue). Data were generated using the standard formulation of this product.
Revision 1
Flow cytometric analysis of Jurkat cells using DRAQ5® #4084 and Cyclin A2 (E6D1J) XP® Rabbit mAb (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 488® Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.