Revision 1
#8462
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
108
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9NQC7
Entrez-Gene Id:
1540
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:200 |
Storage
Specificity/Sensitivity
CYLD (D1A10) Rabbit mAb recognizes endogenous levels of total CYLD protein. This antibody detects an unidentified protein of approximately 28 kDa in some cell types.
Species predicted to react based on 100% sequence homology
Hamster, Bovine, Dog, Horse, Guinea Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys37 of human CYLD protein.
Background
CYLD is a cytoplasmic deubiquitinating enzyme encoded by a tumor suppressor gene altered in individuals diagnosed with cylindromatosis, a genetic condition characterized by benign tumors of skin appendages (1,2). Functional CYLD deubiquitinase regulates inflammation and cell proliferation by down regulating NF-κB signaling through removal of ubiquitin chains from several NF-κB pathway proteins (3,4). CYLD is a negative regulator of proximal events in Wnt/β-catenin signaling and is a critical regulator of natural killer T cell development (5,6). The transcription factor Snail can inhibit CYLD expression, resulting in melanoma cell proliferation (7).
Background References
- Biggs, P.J. et al. (1995) Nat Genet 11, 441-3.
- Sun, S.C. (2010) Cell Death Differ 17, 25-34.
- Regamey, A. et al. (2003) J Exp Med 198, 1959-64.
- Glittenberg, M. and Ligoxygakis, P. (2007) Fly (Austin) 1, 330-2.
- Tauriello, D.V. et al. (2010) Mol Cell 37, 607-19.
- Lee, A.J. et al. (2010) EMBO J 29, 1600-12.
- Massoumi, R. et al. (2009) J Exp Med 206, 221-32.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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Revision 1
#8462
CYLD (D1A10) Rabbit mAb
Western blot analysis of extracts from various cell lines using CYLD (D1A10) Rabbit mAb.
Immunoprecipitation of CYLD protein from 293 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CYLD (D1A10) Rabbit mAb. Western blot analysis was performed using CYLD (D1A10) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used a secondary antibody.