Revision 1
#99979
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
100
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q9NZ08
Entrez-Gene Id:
51752
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity/Sensitivity
ERAP1 (E6X1P) Rabbit mAb recognizes endogenous levels of total ERAP1 protein. This antibody does not cross-react with ERAP2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly840 of human ERAP1 protein.
Background
Endoplasmic reticulum aminopeptidase 1 (ERAP1) is an IFN-γ inducible peptidase that is inserted into the endoplasmic reticulum (ER) membrane as a type II transmembrane protein (1). Research studies have shown that ERAP1 influences the adaptive branch of the immune system by facilitating the maturation of peptide fragments that are loaded onto MHC class I alleles, which are then presented for recognition by T lymphocytes (2,3). Defects in ERAP1 are associated with human disease. Multiple single nucleotide polymorphisms have been identified within ERAP1, which alter the enzymatic activity of the protein product and, in conjunction with HLA-B27, confer susceptibility to ankylosing spondylitis, an inflammatory disease that affects joints (4,5).
Background References
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
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XP is a registered trademark of Cell Signaling Technology, Inc.
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Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#99979
ERAP1 (E6X1P) Rabbit mAb
Western blot analysis of extracts from various human cell lines using ERAP1 (E6X1P) Rabbit mAb.
Western blot analysis of extracts from HeLa and SW620 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #80385 (100 ng/mL, 48 hr; +), using ERAP1 (E6X1P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). ERAP1 protein expression is induced by IFN-γ treatment, as expected.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human ERAP1 (hERAP1-Myc/DDK; +) and Myc/DDK-tagged full-length human ERAP2 (hERAP2-Myc/DDK; +), using ERAP1 (E6X1P) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Revision 1
#99979
ERAP1 (E6X1P) Rabbit mAb
Immunoprecipitation of ERAP1 protein from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ERAP1 (E6X1P) Rabbit mAb. Western blot analysis was performed using ERAP1 (E6X1P) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.