FcγRIII/CD16 (F6K6G) Rabbit mAb #71345

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Supporting Data

REACTIVITY	M R
SENSITIVITY	Endogenous
MW (kDa)	40-60
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

FcγRIII/CD16 (F6K6G) Rabbit mAb recognizes endogenous levels of total mouse FcγRIII/CD16 protein. This antibody does not cross-react with mouse FcγRII/CD32 protein. This antibody detects a 10 kDa band of unknown origin in some cell lines.

Species Reactivity:

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln191 of mouse FcγRIII/CD16 protein.

Background

CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).

Mouse CD16 (FcgammaRIII) is widely expressed across many tissue types and in macrophages, stimulation with IFN-γ increases expression (4). Mouse CD16 has been shown to bind with mouse IgG1, IgG2a, and IgG2b (4).

Database Links

UniProt ID: P08508

Entrez-Gene Id: 14131

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